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ActiPro™ system of media and supplements

HyClone™ ActiPro system of media and supplements is a high-performing solution designed for a robust bioprocess using Chinese Hamster Ovary (CHO) cells. With an optimized and chemically defined formulation, the system allows clones to reach high viable cell densities fast in batch or fed-batch cultures. ActiPro media and supplements are intended for the industrial production of recombinant proteins, including those requiring complex post-translational modifications and correct protein folding.

Catalog # Description
SH31037.01 ActiPro powder medium* - 5 L

$230.00

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Full Specifications
Quantity 5 L bottle
Format Powder
With Additives Poloxamer 188
Without Additives L-glutamine
Sterilization Non-sterile
Storage Requirements 2° to 8°C, away from light and moisture
Shelf Life 18 months
Item Description Powder medium with Poloxamer 188; without L-glutamine
SH31037.02 ActiPro powder medium† - 10 L

$430.00

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Full Specifications
Quantity 10 L bottle
Format Powder
With Additives Poloxamer 188
Without Additives L-glutamine
Sterilization Non-sterile
Storage Requirements 2° to 8°C, away from light and moisture
Shelf Life 18 months
Item Description Powder medium with Poloxamer 188; without L-glutamine
SH31037.05 ActiPro powder medium* - 25 L

$1000.00

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Full Specifications
Quantity 25 L bottle
Format Powder
With Additives Poloxamer 188
Without Additives L-glutamine
Sterilization Non-sterile
Storage Requirements 2° to 8°C, away from light and moisture
Shelf Life 18 months
Item Description Powder medium with Poloxamer 188; without L-glutamine
SH31039.01 ActiPro liquid medium* - 500 mL

$54.00

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Full Specifications
Quantity 500 mL bottle
Format Liquid
With Additives Poloxamer 188
Without Additives L-glutamine
Sterilization 0.2 um sterile filtered
Storage Requirements 2° to 8°C, away from light
Shelf Life 12 months
Item Description Liquid medium with Poloxamer 188; without L-glutamine
SH31039.02 ActiPro liquid medium* - 1000 mL

$90.00

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Full Specifications
Quantity 1000 mL bottle
Format Liquid
With Additives Poloxamer 188
Without Additives L-glutamine
Sterilization 0.2 um sterile filtered
Storage Requirements 2° to 8°C, away from light
Shelf Life 12 months
Item Description Liquid medium with Poloxamer 188; without L-glutamine
SH31039.03 ActiPro liquid medium† - 1 L

$200.00

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Full Specifications
Quantity 1 L poly bag
Format Liquid
With Additives Poloxamer 188
Without Additives L-glutamine
Sterilization 0.2 um sterile filtered
Storage Requirements 2° to 8°C, away from light
Shelf Life 12 months
Item Description Liquid medium with Poloxamer 188; without L-glutamine
SH31038.01 ActiSM powder medium* - 5 L

$300.00

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Full Specifications
Quantity 5 L bottle
Format Powder
With Additives Poloxamer 188
Without Additives L-glutamine
Sterilization Non-sterile
Storage Requirements 2° to 8°C, away from light and moisture
Shelf Life 18 months
Item Description Powder medium with Poloxamer 188; without L-glutamine
SH31038.02 ActiSM powder medium† - 10 L

$600.00

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Full Specifications
Quantity 10 L bottle
Format Powder
With Additives Poloxamer 188
Without Additives L-glutamine
Sterilization Non-sterile
Storage Requirements 2° to 8°C, away from light and moisture
Shelf Life 18 months
Item Description Powder medium with Poloxamer 188; without L-glutamine
SH31038.05 ActiSM powder medium† - 25 L

$1400.00

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Full Specifications
Quantity 25 L bottle
Format Powder
With Additives Poloxamer 188
Without Additives L-glutamine
Sterilization Non-sterile
Storage Requirements 2° to 8°C, away from light and moisture
Shelf Life 18 months
Item Description Powder medium with Poloxamer 188; without L-glutamine
SH31040.01 ActiSM liquid medium* - 500 mL

$49.00

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Full Specifications
Quantity 500 mL bottle
Format Liquid
With Additives Poloxamer 188
Without Additives L-glutamine
Sterilization 0.2 um sterile filtered
Storage Requirements 2° to 8°C, away from light
Shelf Life 12 months
Item Description Powder medium with Poloxamer 188; without L-glutamine
SH31040.02 ActiSM liquid medium* - 1000 mL

$94.50

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Full Specifications
Quantity 1000 mL bottle
Format Liquid
With Additives Poloxamer 188
Without Additives L-glutamine
Sterilization 0.2 um sterile filtered
Storage Requirements 2° to 8°C, away from light
Shelf Life 12 months
Item Description Liquid medium with Poloxamer 188; without L-glutamine
SH31040.03 ActiSM liquid medium† - 1 L

$220.00

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Full Specifications
Quantity 1 L poly bag
Format Liquid
With Additives Poloxamer 188
Without Additives L-glutamine
Sterilization 0.2 um sterile filtered
Storage Requirements 2° to 8°C, away from light
Shelf Life 12 months
Item Description Powder medium with Poloxamer 188; without L-glutamine
SH31026.07 Cell Boost 7a Powder† - 1 L

$200.00

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Full Specifications
Quantity 1 L bottle
Format Powder
With Additives Poloxamer 188
Without Additives L-glutamine
Sterilization Non-sterile
Storage Requirements 2° to 8°C, away from light and moisture
Shelf Life 18 months
Item Description Powder medium with Poloxamer 188; without L-glutamine
SH31026.01 Cell Boost 7a Powder† - 5 L

$900.00

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Full Specifications
Quantity 5 L bottle
Format Powder
With Additives Poloxamer 188
Without Additives L-glutamine
Sterilization Non-sterile
Storage Requirements 2° to 8°C, away from light and moisture
Shelf Life 18 months
Item Description Powder medium with Poloxamer 188; without L-glutamine
SH31026.02 Cell Boost 7a Powder† - 10 L

$1800.00

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Full Specifications
Quantity 10 L bottle
Format Powder
With Additives Poloxamer 188
Without Additives L-glutamine
Sterilization Non-sterile
Storage Requirements 2° to 8°C, away from light and moisture
Shelf Life 18 months
Item Description Powder medium with Poloxamer 188; without L-glutamine
SH31026.03 Cell Boost 7a Powder† - 25 L

$4100.00

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Full Specifications
Quantity 25 L poly bag/pail
Format Powder
With Additives Poloxamer 188
Without Additives L-glutamine
Sterilization Non-sterile
Storage Requirements 2° to 8°C, away from light and moisture
Shelf Life 18 months
Item Description Powder medium with Poloxamer 188; without L-glutamine
SH31027.01 Cell Boost 7b Powder* - 0.5 L

$175.00

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Full Specifications
Quantity 0.5 L bottle
Format Powder
With Additives  
Without Additives L-glutamine, poloxamer 188, insulin
Sterilization Non-sterile
Storage Requirements 2° to 8°C, away from light and moisture
Shelf Life 18 months
Item Description Powder medium with Poloxamer 188; without L-glutamine
SH31027.02 Cell Boost 7b Powder* - 5 L

$1680.00

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Full Specifications
Quantity 5 L bottle
Format Powder
With Additives  
Without Additives L-glutamine
Sterilization Non-sterile
Storage Requirements 2° to 8°C, away from light and moisture
Shelf Life 18 months
Item Description Powder medium with Poloxamer 188; without L-glutamine
SH31027.04 Cell Boost 7b Powder† - 10 L

$3360.00

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Full Specifications
Quantity 10 L bottle
Format Powder
With Additives  
Without Additives L-glutamine, poloxamer 188, insulin
Sterilization Non-sterile
Storage Requirements 2° to 8°C, away from light and moisture
Shelf Life 18 months
Item Description Powder medium with Poloxamer 188; without L-glutamine
SH31027.07 Cell Boost 7b Powder† - 1 L

$300.00

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Full Specifications
Quantity 1 L bottle
Format Powder
With Additives  
Without Additives L-glutamine, poloxamer 188, insulin
Sterilization Non-sterile
Storage Requirements 2° to 8°C, away from light and moisture
Shelf Life 18 months
Item Description Powder medium with Poloxamer 188; without L-glutamine
SH30034.01 L-glutamine, 200 mM solution (29.2 mg/mL L-glutamine in 0.85% NaCl)* - 100 mL

$22.10

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Full Specifications
Quantity 100 mL bottle
Format Liquid
Concentration 29.2 mg/mL
Sterilization 0.1 um sterile filtered
Storage Requirements -5° to -20°C
Shelf Life 12 months
Item Description Liquid; 200 mM in 0.85% NaCl
SH30034.02 L-glutamine, 200 mM solution (29.2 mg/mL L-glutamine in 0.85% NaCl)* - 500 mL

$30.00

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Full Specifications
Quantity 500 mL bottle
Format Liquid
Concentration 29.2 mg/mL
Sterilization 0.1 um sterile filtered
Storage Requirements -5° to -20°C
Shelf Life 12 months
Item Description Liquid; 200 mM in 0.85% NaCl

Note: Powder product quantity is shown as the final volume after powder reconstitution

* Stock items

Item is made to order. Lead times and minimum order quantities apply

Product Overview

The comprehensive HyClone ActiPro system of media and supplements is an innovative cell culture solution designed for high yields in large-scale protein production in batch or fed-batch cultures using recombinant Chinese hamster ovary (CHO) cells. The ActiPro system is chemically defined with no peptides or hydrolysates, and is animal-derived component-free (ADCF). As the ActiPro media and supplements do not contain hypoxanthine or thymidine (HT), the formulation supports the dihydrofolate reductase (DHFR) gene amplification and selection system. This high-level mammalian expression system employs DHFR-deficient CHO cells in a suspension culture with appropriate metabolite compliments. The ActiPro system is intended for the industrial production of recombinant proteins including those requiring complex post-translational modifications and correct protein folding.

 

 Key features of the ActiPro system of media and supplements
  • Ready-to-use starter and scale-up kits

     

  • Chemically defined ADCF formulation

     

  • Does not contain HT, supporting the DHFR system

     

  • Regulatory-friendly

     

  • High-yield production of complex proteins

     

  • Short time to market

     

ActiPro production medium

The medium is used for achieving high yield of active protein during cultivation of recombinant CHO cells. Using ActiPro medium with Cell Boost 7a and 7b supplements in fed-batch or perfusion cultures can contribute to significantly increased cell growth and protein yield.

 

ActiSM adaptation medium

ActiSM medium allows the cultivation or adaptation of recombinant CHO cells in small-scale shaker flasks or spinner bottles. This adaptation medium may also be used while working with methotrexate. The lean formulation of ActiSM medium can help simplify and shorten the time-consuming adaptation to fed-batch culture.

 

Cell Boost 7a and Cell Boost 7b feed supplements

Cell Boost 7a and 7b supplements are used as an essential part of a cell culture feed strategy to enhance cell culture performance and increase product yield. Cell Boost 7a and 7b are intended for use together in defined concentrations. Cell Boost 7a and 7b are feed additives especially matched to ActiPro production medium. Combining Cell Boost 7a and 7b has demonstrated significantly increased productivity when compared with batch culture. Established protocols facilitate easy and efficient identification of a feeding strategy. Cell Boost 7a contains concentrated amino acids, vitamins, salts, trace elements, and a carbon source. Cell Boost 7b is a balanced formulation of selected amino acids in a concentrated form.

 

High flexibility with the ActiPro system of media and supplements

Use of the complete ActiPro system of media and supplements helps accelerate the time-consuming fed-batch optimization process. For fast-growing CHO cells with higher carbon consumption, glucose supplementation is needed.